But the military leadership in Misrata, where Kadhafi's body had been stored in a vegetable market freezer overnight and was again put on display for hundreds of curious onlookers on Saturday, insisted the inquiry would involve no autopsy.
"There will be no post-mortem today, nor any day," Misrata military council spokesman Fathi al-Bashaagha told AFP. "No one is going to open up his body."
His comments were confirmed by two other Misrata military chiefs.
Bashaagha said the new regime's military commander for the capital, Abdelhakim Belhaj, was expected to travel to Misrata later on Saturday to view the corpse of the man who ruled Libya with an iron rod for 42 years.
But he said there were no immediate plans for National Transitional Council (NTC) chief Abdel Jalil to visit.
"Abdel Jajil did not come yesterday and is not coming today, and for the moment it is not expected that he will come."
The interim leader was in the main eastern city of Benghazi on Saturday visiting some of the wounded from the eight-month uprising that felled Kadhafi and preparing for a ceremony there on Sunday to proclaim the country totally liberated.
"Yes," he answered when asked if the circumstances of Kadhafi's death were being investigated. He declined to take any further questions.
As the rigid, bloodied, yellow corpses of Kadhafi and his son Mutassim lay on dirty mattresses on the metal floor of the glacial makeshift mortuary, hundreds of Libyans were allowed inside to view them.
A man identifying himself as Sadiq said he was only 18 when the former despot took power in 1969.
"All my adult life I lived with this low life, this..." said the 60-year-old, who declined to give his last name, spattering curses against Kadhafi.
"But he is dead and I am happy," he said, laughing.
US President Barack Obama said that "in Libya, the death of Moamer Kadhafi showed that our role in protecting the Libyan people, and helping them break free from a tyrant, was the right thing to do."
But questions remain over how Kadhafi met his end after NTC fighters hauled him out of a culvert where he was hiding following NATO air strikes on the convoy in which he had been trying to flee his falling hometown.
Mobile phone videos show him still alive at that point.
Footage shows the former dictator, his face half-covered in blood, being dragged towards a vehicle by a delirious crowd and forced on to the bonnet.
Those at the front push and shake him, pull him by the hair and hit him. At one point he appears to be trying to speak.
Subsequent footage shows him being hauled off the vehicle, still alive, and hustled through the screaming crowd, before he disappears in the crush and the crackle of gunfire can be heard.
NTC leaders are adamant he was shot in the head when he was caught "in crossfire" between his supporters and new regime fighters soon after his capture.
Russian Foreign Minister Sergei Lavrov said the "way his death happened poses an entire number of questions," and called for a probe.
"The images we saw on television show that he was taken prisoner while wounded, and then later, once already a prisoner, his life was taken away."
US State Department spokesman Mark Toner said the NTC "has already been working to determine the precise cause and circumstances of Kadhafi's death, and we obviously urge them to do so in an open and transparent manner as we move forward."
Toner repeated a US call to the NTC to treat prisoners humanely.
UN human rights chief Navi Pillay also called for an investigation.
"On the issue of Kadhafi's death yesterday, the circumstances are still unclear," her spokesman Rupert Colville said. "There should be some kind of investigation given what we saw yesterday."
Claudio Cordone, senior director at Amnesty International, said that if Kadhafi "was killed after his capture, it would constitute a war crime and those responsible should be brought to justice."
He said the "NTC must apply the same standards to all, affording justice even to those who categorically denied it to others."
And Kadhafi's widow, Safia, who fled to Algeria in August, called on the United Nations to investigate the circumstances of her husband's death, Syria-based Arrai television said.
Libya's wanted former intelligence chief Abdullah al-Senussi, meanwhile, surfaced in neighbouring Niger after apparently fleeing through the desert following the fall of the oasis town of Bani Walid on Monday in the penultimate battle of the conflict.
"The presence of Abdullah al-Senussi has been indicated in the far north of Niger," near the Libyan border, a government source in the capital Niamey told AFP on Saturday.
"It's a report that we have, but our defence and security forces have not yet intercepted him," the source said. "So his presence in Niger is not yet officially established."
Senussi is wanted by the International Criminal Court on charges of "crimes against humanity." The court leveled the same charges against Kadhafi and his son and longtime heir apparent Seif al-Islam.
Seif al-Islam too remains at large. NTC officials said that he too may have fled to Niger.
At a World Economic Forum in Jordan, Libya's interim prime minister Mahmud Jibril warned on Saturday that rebuilding his war-battered country would amount to a "Mission Impossible" task.
"Rebuilding Libya will not be an easy task. It is Mission Impossible of Tom Cruise," Jibril said, referring to the Hollywood star's movie.
And Swiss President Micheline Calmy-Rey offered her country's help in disarming the population and in demining efforts.
In an interview with newspaper Sonntag, she also expressed hopes that some 265 million francs (216 million euros, $300 million) in blocked Libyan assets could soon be released.
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